Influence of Ca2+e on 5-HT2- and alpha 1-induced arterial contraction and phosphoinositide metabolism

Abstract

Serotonin and phenylephrine were found to induce contractile responses and inositol phosphate (IP) formation in isolated rat tail artery. Both processes displayed similar concentration dependence in the presence of 2.5 mM external calcium (Ca2+e) and both were respectively inhibited by either ketanserine or prazosin, depending on the agonist used. In the absence of Ca2+e the amines no longer produced a contractile effect. In addition, lack of Ca2+ caused a shift to the right in the dose-response curve for phenylephrine-induced IP formation whereas serotonin-induced IP formation was not affected by changes in Ca2+e. The results suggest that alpha 1- and 5-HT2-induced contractions are quantitatively related to phosphatidylinositol metabolism. Contraction, but not IP formation requires the presence of Ca2+e. Different effects of Ca2+e on phenylephrine- and serotonin-induced IP formation could be related to a differential Ca2+ effect on binding of alpha 1- or 5-HT2 agonists.