Uptake and transformation of steroid estrogens as emerging contaminants influence plant development

Abstract

Steroid estrogens are emerging contaminants of concern due to their devastating effects on reproduction and development in animals and humans at very low concentrations. The increasing steroid estrogen in the environment all over the world contrasts very few studies for potential impacts on plant development as a result of estrogen uptake. This study evaluated the uptake, transformation and effects of estradiol (17β-E2) and ethinyl estradiol (EE2) (0.1-1000 μg L^-1) on lettuce. Uptake increased in leaves and roots in a dose-dependent manner, and roots were the major organ in which most of the estrogen was deposited. The transformation of estrogens to major metabolite and their further reverse biotransformation in lettuce tissue was identified. At low concentrations (0.1 and 50 μg L^-1) estrogens resulted in enhanced photosynthetic pigments, root growth and shoot biomass. Application of higher concentrations of estrogens (10 mg L^-1) significantly reduced total root growth and development. This was accompanied by increased levels of hydrogen peroxide (H₂O₂), and malondialdehyde (MDA), and activities of antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX). Taken together, these findings suggest that at low concentrations estrogens may biostimulate growth and primary metabolism of lettuce, while at elevated levels they have adverse effects.

Keywords: Antioxidant system; Bioavailability; Biotransformation; Estrogens; Plant uptake.