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. 2017 Nov;24(7):1679-1688.
doi: 10.1016/j.sjbs.2015.11.008. Epub 2015 Nov 24.

Superintendence of antimicrobial resistance observed in bacterial flora isolated from human faecal carriage in Vellore, India

Sundera Raj Esther Priyadharshini  1 , Chidamabram Ramalingam  1 , Balasubramanian Ramesh  2
Affiliations

Superintendence of antimicrobial resistance observed in bacterial flora isolated from human faecal carriage in Vellore, India

Sundera Raj Esther Priyadharshini et al. Saudi J Biol Sci. 2017 Nov.

Abstract

A frequent cross-sectional study was conducted to determine the patterns of antimicrobial resistance in 296 bacterial strains isolated from in-patient faecal samples of Government Vellore Medical College and Hospital, Vellore. Isolation and identification of bacterial strains were done using enrichment media, selective media, and biochemical tests. Antimicrobial susceptibility testing by the disc diffusion method and minimal inhibitory concentration method was conducted and the strains were subjected to extended spectrum beta-lactamases screening. Antibiotic sensitivity pattern of Staphylococcus spp. showed oxacillin resistance. Almost all the strains were sensitive to linezolid, vancomycin, gentamycin and chloramphenicol. In gram negative isolates ciprofloxacin and tobramycin showed better sensitivity and ceftazidime showed a higher percentage of resistance by MIC. Out of 250 isolates, Enterobacteriaceae showed positive for 86/250, 82/250 and 94/250 isolates and 3/10, 4/10 and 4/10 non-Enterobacteriaceae isolates were found to be positive for CTX-M gene, TEM gene and SHV gene, respectively. This study helps to assess/analyse the relation between the spectrum of microorganisms present in various grades of faecal carriage and their susceptibility pattern in this part of the Vellore town.

Keywords: Bacteria; ESBL; Faecal carriage; Vellore.

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Figures

Figure 1
Figure 1
Agarose gel showing products of CTX-M PCR amplification.
Figure 2
Figure 2
Agarose gel showing products of TEM PCR amplification.
Figure 3
Figure 3
Agarose gel showing products of SHV PCR amplification.
See this image and copyright information in PMC

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