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. 1987 Jan 26;15(2):771-84.
doi: 10.1093/nar/15.2.771.

DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy

T Adachi, M Mizuuchi, E A Robinson, E Appella, M H O'Dea, M Gellert, K Mizuuchi
Free PMC article

DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy

T Adachi et al. Nucleic Acids Res. .
Free PMC article

Abstract

We have determined the sequence of the E. coli gyrB gene, using a new sequencing approach in which transposition from a mini-Mu plasmid into the DNA provides random start points for dideoxynucleotide sequence analysis. The gyrB sequence corresponds to a protein 804 amino acids long; a previously isolated protein fragment with partial enzymatic activity has been identified as the C-terminal half-molecule. A plausible terminator of gyrB transcription is located just beyond the structural gene.

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References

    1. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4767-71 - PubMed
    1. Annu Rev Genet. 1986;20:385-429 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1773-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5960-3 - PubMed
    1. Mol Gen Genet. 1979 Sep;175(2):135-44 - PubMed

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