Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1987 Feb 11;15(3):1005-17.
doi: 10.1093/nar/15.3.1005.

Cloning and characterization of the gene for Escherichia coli seryl-tRNA synthetase

M Härtlein, D Madern, R Leberman
Free PMC article
Comparative Study

Cloning and characterization of the gene for Escherichia coli seryl-tRNA synthetase

M Härtlein et al. Nucleic Acids Res. .
Free PMC article

Abstract

Seryl-tRNA synthetase is the gene product of the serS locus in Escherichia coli. Its gene has been cloned by complementation of a serS temperature sensitive mutant K28 with an E. coli gene bank DNA. The resulting clones overexpress seryl-tRNA synthetase by a factor greater than 50 and more than 6% of the total cellular protein corresponds to the enzyme. The DNA sequence of the complete coding region and the 5'- and 3' untranslated regions was determined. Protein sequence comparison of SerRS with all available aminoacyl-tRNA synthetase sequences revealed some regions of significant homology particularly with the isoleucyl- and phenylalanyl-tRNA synthetases from E. coli.

PubMed Disclaimer

References

    1. FEBS Lett. 1980 Oct 6;119(2):201-11 - PubMed
    1. Biochimie. 1986 Sep;68(9):1071-8 - PubMed
    1. J Mol Biol. 1982 Feb 15;155(1):63-81 - PubMed
    1. J Biol Chem. 1982 Oct 10;257(19):11644-50 - PubMed
    1. J Mol Biol. 1982 Jul 15;158(4):699-709 - PubMed

Publication types

Associated data