Telomeric site position heterogeneity in macronuclear DNA of Paramecium primaurelia
- PMID: 3029725
- PMCID: PMC340576
- DOI: 10.1093/nar/15.4.1717
Telomeric site position heterogeneity in macronuclear DNA of Paramecium primaurelia
Abstract
In Paramecium primaurelia, the macronuclear gene encoding the G surface protein is located near a telomere. In this study, multiple copies of this telomere have been isolated and the subtelomeric and telomeric regions of some of them have been sequenced. The telomeric sequences consist of tandem repeats of the hexanucleotides C4A2 or C3A3. We show that the location where these repeats are added, which we call the telomeric site, is variable within a 0.6-0.8-kb region. These results are discussed in relation with the formation of macronuclear DNA.
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