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. 2019 Jun;43(6):1295-1304.
doi: 10.1038/s41366-018-0224-9. Epub 2018 Oct 9.

High-fat diet consumption alters energy metabolism in the mouse hypothalamus

Blanca Lizarbe  1 , Antoine Cherix  2 , João M N Duarte  2   3   4 , Jean-René Cardinaux  5 , Rolf Gruetter  2   6   7
Affiliations

High-fat diet consumption alters energy metabolism in the mouse hypothalamus

Blanca Lizarbe et al. Int J Obes (Lond). 2019 Jun.

Abstract

Background/objectives: High-fat diet consumption is known to trigger an inflammatory response in the hypothalamus, which has been characterized by an initial expression of pro-inflammatory genes followed by hypothalamic astrocytosis, microgliosis, and the appearance of neuronal injury markers. The specific effects of high-fat diet on hypothalamic energy metabolism and neurotransmission are however not yet known and have not been investigated before.

Subjects/methods: We used 1H and 13C magnetic resonance spectroscopy (MRS) and immunofluorescence techniques to evaluate in vivo the consequences of high-saturated fat diet administration to mice, and explored the effects on hypothalamic metabolism in three mouse cohorts at different time points for up to 4 months.

Results: We found that high-fat diet increases significantly the hypothalamic levels of glucose (P < 0.001), osmolytes (P < 0.001), and neurotransmitters (P < 0.05) from 2 months of diet, and alters the rates of metabolic (P < 0.05) and neurotransmission fluxes (P < 0.001), and the contribution of non-glycolytic substrates to hypothalamic metabolism (P < 0.05) after 10 weeks of high-fat feeding.

Conclusions/interpretation: We report changes that reveal a high-fat diet-induced alteration of hypothalamic metabolism and neurotransmission that is quantifiable by 1H and 13C MRS in vivo, and present the first evidence of the extension of the inflammation pathology to a localized metabolic imbalance.

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References

    1. James WP. WHO recognition of the global obesity epidemic. Int J Obes. 2008;32(Suppl 7):S120–6. - DOI
    1. Nilsson C, Raun K, Yan FF, Larsen MO, Tang-Christensen M. Laboratory animals as surrogate models of human obesity. Acta Pharmacol Sin. 2012;33:173–81. - PubMed - PMC - DOI
    1. Morton GJ, Cummings DE, Baskin DG, Barsh GS, Schwartz MW. Central nervous system control of food intake and body weight. Nature. 2006;443:289–95. - PubMed - DOI
    1. Karnani M, Burdakov D. Multiple hypothalamic circuits sense and regulate glucose levels. Am J Physiol Regul Integr Comp Physiol. 2011;300:R47–55. - PubMed - DOI
    1. Lam TK, Pocai A, Gutierrez-Juarez R, Obici S, Bryan J, Aguilar-Bryan L, et al. Hypothalamic sensing of circulating fatty acids is required for glucose homeostasis. Nat Med. 2005;11:320–7. - PubMed - DOI

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