Hormonal control of fructose 2,6-bisphosphate concentration in the HT29 human colon adenocarcinoma cell line. Alpha 2-adrenergic agonists counteract effect of vasoactive intestinal peptide
- PMID: 3030263
- PMCID: PMC1147319
- DOI: 10.1042/bj2390531
Hormonal control of fructose 2,6-bisphosphate concentration in the HT29 human colon adenocarcinoma cell line. Alpha 2-adrenergic agonists counteract effect of vasoactive intestinal peptide
Abstract
Vasoactive intestinal peptide (VIP) was found to cause a dose-dependent decrease in fructose 2,6-bisphosphatase concomitant with an increase in cyclic AMP in cultured HT29 cancer cells from human colon. The maximum effect was a 41% decrease obtained with 10 nM-VIP, and half-maximum effect was obtained with 0.75 nM-VIP. The effect of 2.5 nM-VIP was almost totally counteracted (i.e. fructose 2,6-bisphosphate concentration was restored) by either adrenaline (1 microM) or the alpha 2-adrenergic agonist UK-14304 (1 microM); the alpha 2-agonist clonidine (1 microM) was less efficient, since the VIP effect was decreased by 72% only. The adrenaline effect was totally antagonized by 1 microM-yohimbine. It is concluded that, in the HT29 cancer cells, the fructose 2,6-bisphosphate-producing system is sensitive to variations of cyclic AMP concentration and is under the dual control of VIP and alpha 2-adrenergic receptors.
Similar articles
-
alpha 2-Adrenoceptors in the HT 29 human colon adenocarcinoma cell line: characterization with [3H]clonidine; effects on cyclic AMP accumulation.Eur J Pharmacol. 1985 Jan 2;107(2):223-31. doi: 10.1016/0014-2999(85)90062-7. Eur J Pharmacol. 1985. PMID: 2984004
-
Inhibition of cyclic AMP-dependent chloride secretion by PP receptors and alpha 2-adrenoceptors in a human colonic epithelial cell line.Naunyn Schmiedebergs Arch Pharmacol. 1997 Feb;355(2):183-9. doi: 10.1007/pl00004930. Naunyn Schmiedebergs Arch Pharmacol. 1997. PMID: 9050010
-
Adrenergic versus VIPergic control of cyclic AMP in human colonic crypts.Peptides. 1984 Mar-Apr;5(2):379-83. doi: 10.1016/0196-9781(84)90238-9. Peptides. 1984. PMID: 6147817
-
Interactions of full and partial agonists with HT29 cell alpha 2-adrenoceptor: comparative study of [3H]UK-14,304 and [3H]clonidine binding.Mol Pharmacol. 1989 Mar;35(3):345-54. Mol Pharmacol. 1989. PMID: 2564632
-
Alpha 2-adrenergic receptors in the human cell line, HT29. Characterization with the full agonist radioligand [3H]UK-14,304 and inhibition of adenylate cyclase.Mol Pharmacol. 1985 Nov;28(5):422-30. Mol Pharmacol. 1985. PMID: 2865671
Cited by
-
An inducible gene product for 6-phosphofructo-2-kinase with an AU-rich instability element: role in tumor cell glycolysis and the Warburg effect.Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):3047-52. doi: 10.1073/pnas.96.6.3047. Proc Natl Acad Sci U S A. 1999. PMID: 10077634 Free PMC article.
-
Insulin controls key steps of carbohydrate metabolism in cultured HT29 colon cancer cells.Biochem J. 1989 Jul 1;261(1):175-9. doi: 10.1042/bj2610175. Biochem J. 1989. PMID: 2505753 Free PMC article.
-
Coupling of the alpha 2-adrenergic receptor to the inhibitory G-protein Gi and adenylate cyclase in HT29 cells.Biochem J. 1993 May 15;292 ( Pt 1)(Pt 1):283-8. doi: 10.1042/bj2920283. Biochem J. 1993. PMID: 8099279 Free PMC article.
-
Questions about the use of [3H]thymidine incorporation as a reliable method to estimate cell proliferation rate.Biochem J. 1990 Sep 1;270(2):563-4. doi: 10.1042/bj2700563b. Biochem J. 1990. PMID: 2400404 Free PMC article. No abstract available.
-
Fructose 2,6-bisphosphate metabolism in Ehrlich ascites tumour cells.J Cancer Res Clin Oncol. 1995;121(12):739-45. doi: 10.1007/BF01213320. J Cancer Res Clin Oncol. 1995. PMID: 7499445 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
