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. 1987 Apr;14(3):305-16.
doi: 10.1016/0165-5728(87)90017-8.

Analysis of the intrathecal humoral immune response in Brown Norway (BN) rats, infected with the murine coronavirus JHM

R Dörries, R Watanabe, H Wege, V ter Meulen

Analysis of the intrathecal humoral immune response in Brown Norway (BN) rats, infected with the murine coronavirus JHM

R Dörries et al. J Neuroimmunol. 1987 Apr.

Abstract

Serum and CSF specimens from clinically healthy Brown Norway (BN) rats inoculated intracerebrally with corona virus JHM were analysed with respect to the state of the blood-brain barrier (BBB) and the intrathecal synthesis and isoelectric distribution of immunoglobulins (Ig). Increased CSF/serum ratios for Ig in the context of an intact BBB were never seen in the absence of intrathecal synthesis of virus-specific antibodies. Affinity-mediated immunoblot analysis revealed a broad pattern of virus-specific antibodies with embedded clusters of restricted heterogeneity, but no signs of oligoclonal Ig production carrying non-viral specificity. From these data it was concluded that BN rats do control the intracerebral spread of JHM virus effectively by a strong local virus-specific antibody response, thereby preventing a clinically apparent disease.

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References

    1. Arnadottir T., Reunanen M., Salmi A. Intrathecal synthesis of virus antibodies in multiple sclerosis patients. Infect. Immun. 1982;38:399–407. - PMC - PubMed
    1. Dörries R., ter Meulen V. Detection and identification of virus-specific oligoclonal IgG in unconcentrated cerebrospinal fluid by immunoblot technique. J. Neuroimmunol. 1984;7:77–89. - PubMed
    1. Dörries R., Watanabe R., Wege H., ter Meulen V. Murine coronavirus-induced encephalomyelitides in rats: analysis of immunoglobulins and virus-specific antibodies in serum and cerebrospinal fluid. J. Neuroimmunol. 1986;12:131–142. - PMC - PubMed
    1. Janeway C.A., Bottomly K., Babich J., Conrad P., Conzen S., Jones B., Kaye J., Katz M., McVay L., Murphy D.B., Tite J. Quantitative variation in Ia antigen expression plays a central role in immune regulation. Immunol. Today. 1984;5:99–105. - PubMed
    1. Johnson R.T. Viral Infections of the Nervous System. Raven Press; New York: 1982. Chronic inflammatory and demyelinating diseases; pp. 244–255. Chap. 10.

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