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. 2019 Feb 23;219(6):867-876.
doi: 10.1093/infdis/jiy599.

Proteomic Profile Associated With Loss of Spontaneous Human Immunodeficiency Virus Type 1 Elite Control

Esther Rodríguez-Gallego  1 , Laura Tarancón-Diez  2 , Felipe García  3 , Jorge Del Romero  4 , Jose Miguel Benito  5 , Verónica Alba  1 , Pol Herrero  6 , Anna Rull  1 , Beatriz Dominguez-Molina  2 , Onofre Martinez-Madrid  7 , Luisa Martin-Pena  8   9 , Federico Pulido  10 , Agathe León  3 , Carmen Rodríguez  4 , Norma Rallón  5 , Joaquim Peraire  1 , Consuelo Viladés  1 , Manuel Leal  2   11 , Francesc Vidal  1 , Ezequiel Ruiz-Mateos  2 ; HIV-1 Elite Controllers Study Group (ECRIS), Spanish AIDS Research Network
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Free article

Proteomic Profile Associated With Loss of Spontaneous Human Immunodeficiency Virus Type 1 Elite Control

Esther Rodríguez-Gallego et al. J Infect Dis. .
Free article

Erratum in

  • Erratum.
    [No authors listed] [No authors listed] J Infect Dis. 2019 Feb 23;219(6):1011. doi: 10.1093/infdis/jiy722. J Infect Dis. 2019. PMID: 30624629 No abstract available.

Abstract

Background: Elite controllers (ECs) spontaneously control plasma human immunodeficiency virus type 1 (HIV-1) RNA without antiretroviral therapy. However, 25% lose virological control over time. The aim of this work was to study the proteomic profile that preceded this loss of virological control to identify potential biomarkers.

Methods: Plasma samples from ECs who spontaneously lost virological control (transient controllers [TCs]), at 2 years and 1 year before the loss of control, were compared with a control group of ECs who persistently maintained virological control during the same follow-up period (persistent controllers [PCs]). Comparative plasma shotgun proteomics was performed with tandem mass tag (TMT) isobaric tag labeling and nanoflow liquid chromatography coupled to Orbitrap mass spectrometry.

Results: Eighteen proteins exhibited differences comparing PC and preloss TC timepoints. These proteins were involved in proinflammatory mechanisms, and some of them play a role in HIV-1 replication and pathogenesis and interact with structural viral proteins. Coagulation factor XI, α-1-antichymotrypsin, ficolin-2, 14-3-3 protein, and galectin-3-binding protein were considered potential biomarkers.

Conclusions: The proteomic signature associated with the spontaneous loss of virological control was characterized by higher levels of inflammation, transendothelial migration, and coagulation. Galectin-3 binding protein could be considered as potential biomarker for the prediction of virological progression and as therapeutic target in ECs.

Keywords: HIV-1; biomarkers; elite controllers; loss of control; proteomic profile.

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