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. 2019 Feb;33(2):e22692.
doi: 10.1002/jcla.22692. Epub 2018 Oct 15.

Global expression profiling of metabolic pathway-related lncRNAs in human gastric cancer and the identification of RP11-555H23.1 as a new diagnostic biomarker

Xiaoyan Mo  1 , Yuanyuan Wu  1 , Li Chen  1 , Ming Zhai  1 , Zhengdong Gao  1 , Kainan Hu  1 , Junming Guo  1
Affiliations

Global expression profiling of metabolic pathway-related lncRNAs in human gastric cancer and the identification of RP11-555H23.1 as a new diagnostic biomarker

Xiaoyan Mo et al. J Clin Lab Anal. 2019 Feb.

Abstract

Background: Long noncoding RNAs (lncRNAs) have roles in regulating metabolism; however, the global expression profile of metabolic pathway-associated lncRNAs in gastric cancer is unknown. The purpose of our study was to examine metabolic pathway-related lncRNAs in gastric cancer and their possible diagnostic values.

Methods: Differential expression patterns of metabolic pathway-related lncRNAs between gastric cancer and paired nontumor tissues were detected using metabolic pathway-associated lncRNA microarrays. The expression of RP11-555H23.1, one representative metabolic pathway-associated lncRNA, was validated using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). The associations between RP11-55H23.1 expression and the clinicopathological features of gastric cancer patients were analyzed. A receiver operating characteristic (ROC) curve was further established.

Results: A total of 114 differentially expressed metabolic pathway-associated lncRNAs (fold change >2, P < 0.05) between cancer and nontumor tissues were found (GEO No. GSE96856). Among them, TUG1, RP11-555H23.1, RP1-257I20.13, UGP2, GCSHP3, and XLOC_000889 lncRNAs were downregulated more than sixfold in gastric cancer tissues. In contrast, RP11-605F14.2, TBC1D3P5, BC130595, LINC00475, RP11-19P22.6, BC080653, XLOC_004923, AFAP1-AS1, EPB49, and RP11-296I10.3 lncRNAs were upregulated more than sixfold in gastric cancer tissues. We further demonstrated that RP11-555H23.1 expression was significantly correlated with TNM stage (P = 0.038). The area under the ROC curve (AUC) was 0.65, and the specificity and sensitivity were 62% and 81%, respectively.

Conclusions: Metabolic pathway-associated lncRNAs play an important role in the occurrence of gastric cancer, and metabolic pathway-associated lncRNAs, such as RP11-555H23.1, may represent novel biomarkers of gastric cancer.

Keywords: RP11-555H23.1; biomarker; gastric cancer; long noncoding RNA; metabolic pathway.

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Conflict of interest statement

None.

Figures

Figure 1
Figure 1
Expression of RP11‐555H23.1 in gastric cancer tissues and paired adjacent normal tissues. RP11‐555H23.1 and GAPDH mRNA levels were measured by qRT‐PCR. ΔC q was used to show relative expression of RP11‐555H23.1 using ΔC q = C q(RP11‐555H23.1) − C q(GAPDH). Small ΔC q indicates higher expression. n = 104, P<0.001
Figure 2
Figure 2
The ROC curve of RP11‐555H23.1
See this image and copyright information in PMC

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