doi: 10.1016/0006-291x(87)90338-x.
The effect of polymyxin B nonapeptide (PMBN) on transformation
- PMID: 3032189
- DOI: 10.1016/0006-291x(87)90338-x
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The effect of polymyxin B nonapeptide (PMBN) on transformation
Biochem Biophys Res Commun.
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Abstract
The effect of the outer membrane permeabilizing polycation, polymyxin B nonapeptide (PMBN) on the transformation of E. coli HB101 with pBR322 plasmid DNA was investigated. Pretreatment of cells with PMBN (followed by suspending the cells in PMBN-free medium) did not stimulate the development of competence induced by the calcium heat pulse. In the absence of calcium-ions, a high PMBN concentration (1 mM) was able to induce a low transformation frequency provided that PMBN was not removed before the addition of DNA.
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