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. 2018 Nov 26;62(12):e01520-18.
doi: 10.1128/AAC.01520-18. Print 2018 Dec.

Genomic Characterization of Carbapenemase-Producing Klebsiella pneumoniae with Chromosomally Carried blaNDM-1

Noriko Sakamoto  1 , Yukihiro Akeda  2   3   4 , Yo Sugawara  1 , Dan Takeuchi  1 , Daisuke Motooka  5 , Norihisa Yamamoto  1   3   4 , Warawut Laolerd  6 , Pitak Santanirand  6 , Shigeyuki Hamada  1
Affiliations

Genomic Characterization of Carbapenemase-Producing Klebsiella pneumoniae with Chromosomally Carried blaNDM-1

Noriko Sakamoto et al. Antimicrob Agents Chemother. .

Abstract

We report here Klebsiella pneumoniae strains carrying chromosomal blaNDM-1 in Thailand. The genomes of these two isolates include a 160-kbp insertion containing blaNDM-1, which is almost identical to that in the IncHI1B-like plasmid. Further analysis indicated that IS5-mediated intermolecular transposition and Tn3 transposase-mediated homologous recombination resulted in the integration of blaNDM-1 into the chromosome from an IncHI1B-like plasmid. The spread of this type of carbapenem-resistant Enterobacteriaceae may threaten public health and warrants further monitoring.

Keywords: IS5; Klebsiella pneumoniae; Tn3; beta-lactamases; blaNDM; carbapenemase-producing Enterobacteriaceae; carbapenems.

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Figures

FIG 1
FIG 1
Comparison of chromosomal 160-kbp regions containing blaNDM-1 in strains KP64 and KP67 and blaNDM-1-carrying plasmid pKP3301 from strain KP33. The gray-shaded area indicates the regions with high identity (>99% similarity) between the two sequences. The 160-kbp region containing blaNDM-1 is highlighted with a red box. Nucleotide letters below the sequences of the KP64 and KP67 chromosomes represent target site duplications.
FIG 2
FIG 2
Hypothetical mechanism of integration of the 160-kbp region of plasmid pKP3301 into the KP64 and KP67 chromosomes. (A) Schematic view of the chromosome of strain KP33 carrying an IS5 copy at the putative insertion site in the 160-kbp region. (B) Plasmid pKP3301 containing blaNDM-1, orf9, and a 4-bp target site (AATG) in the middle of the orf9 gene. (C) IS5 on the chromosome of strain KP33 recognizes the 4-bp target site in orf9, intermolecular transposition generates a cointegrate molecule, and the IncHI1B-like plasmid moiety is truncated (dashed line) by Tn3 transposase-mediated homologous recombination. (D) Generation of the 160-kbp region bracketed by IS5 copies. Purple boxes represent homologous regions among different strains.
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