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. 2018 Oct;7(1):16-22.

Lack of Association between Interleukin 23R (IL-23R) rs10889677 Polymorphism and Inflammatory Bowel Disease Susceptibility In an Iranian Population

Sara Karimkhani  1 , Vahid Chaleshi  1 , Hedieh Balaii  1 , Peyman Tarban  1 , Mahyar Nourian  1 , Shiva Irani  2 , Shabnam Shahrokh  3 , Hamid Asadzadeh Aghdaei  1 , Amir Houshang Mohammad Alizadeh  3 , Mohsen Norouzinia  3 , Mohammad Reza Zali  3
Affiliations

Lack of Association between Interleukin 23R (IL-23R) rs10889677 Polymorphism and Inflammatory Bowel Disease Susceptibility In an Iranian Population

Sara Karimkhani et al. Rep Biochem Mol Biol. 2018 Oct.

Abstract

Background: Inflammatory bowel diseases (IBDs), which include ulcerative colitis (UC) and Crohn's disease (CD), are inflammatory disorders that affect the gastrointestinal tract. A combination of inflammatory cytokines has an important role in IBD development. Genome-wide association studies have shown that polymorphisms in the interleukin-23R gene (IL-23R) increase susceptibility to IBD. The aim of this study was to investigate the IL-23R 3' UTR SNP to determine a potential association between genotype distribution and IBD.

Methods: The case group included 102 IBD patients and the control group included 107 healthy individuals. IL-23R polymorphisms rs10889677 were genotyped using PCR-RFLP analysis. RFLP results were confirmed by direct sequencing.

Results: The allele and genotype frequencies in patients and controls were evaluated and compared, and no significant association between this functional rs10889677 polymorphism and risk of IBD was observed (P=0.587; adjusted OR: 0.89; 95% CI: 0.597-1.339). We also found no significant association between CD (14.71%) and UC (85.29%) patients in allele or genotype levels (P>0.05).

Conclusion: Our results suggest that the rs10889677 A>C polymorphism is not a potential prognostic marker in Iranian patients with IBD.

Keywords: Crohn’s disease; Inflammatory bowel diseases; Interleukin 23 receptor; Ulcerative colitis; rs10889677.

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Figures

Fig. 1
Fig. 1
Agarose gels of PCR products before (A) and after (B) digestion with MnlI. A 50 base-pair marker was used (L50). A) PCR products from samples 84 – 88. B) PCR products after digestion with MnlI. Samples 54 and 84 are from CC genotype subjects, samples 52, 80, and 70 are from AC genotype subjects, and samples 77 and 65 are from AA genotype subjects.
See this image and copyright information in PMC

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