Induction and Readout of Oxygen-Induced Retinopathy

Abstract

Ischemic retinal diseases can be caused by various pathologies, which often lead to formation of preretinal neovascularization. A very common and well-established model to study normal as well as pathological angiogenic mechanisms in retina is the oxygen-induced retinopathy model in mice. This model is based on oxygen exposure of mouse pups during retinal vascular development, leading to a depletion of retinal capillaries. Upon return to room air, the lack of retinal vasculature results in hypoxia, which in turn induces vascular repair and preretinal neovascularization. In this review, we will focus on the scientific options, practical implementation, and quantification of the OIR model and its potential pitfalls.

Keywords: Angiogenesis; Hyperoxia; Oxygen-induced retinopathy; Preretinal neovascularization; Retinopathy of prematurity; Vaso-obliteration; Vessel regrowth.