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. 1987 May;169(5):1824-9.
doi: 10.1128/jb.169.5.1824-1829.1987.

Molecular cloning of a recA-like gene from the cyanobacterium Anabaena variabilis

G W Owttrim, J R Coleman

Molecular cloning of a recA-like gene from the cyanobacterium Anabaena variabilis

G W Owttrim et al. J Bacteriol. 1987 May.

Abstract

A recA-like gene isolated from the cyanobacterium Anabaena variabilis was cloned and partially characterized. When introduced into Escherichia coli recA mutants, the 7.5-kilobase-pair plasmid-borne DNA insert restored resistance to methyl methanesulfonate and UV irradiation, as well as recombination proficiency when measured by Hfr-mediated conjugation. The cyanobacterial recA gene restored spontaneous but not mitomycin C-induced prophage production. Restriction analysis and subcloning yielded a 1.5-kilobase-pair Sau3A fragment which also restored methylmethane sulfonate resistance and coded for a 38- to 40-kilodalton polypeptide when expressed in an in vitro transcription-translation system.

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References

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