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Comparative Study
. 1987 May:68 ( Pt 5):1351-9.
doi: 10.1099/0022-1317-68-5-1351.

Identification of human papillomavirus type 18 E6 polypeptide in cells derived from human cervical carcinomas

L Banks, P Spence, E Androphy, N Hubbert, G Matlashewski, A Murray, L Crawford
Comparative Study

Identification of human papillomavirus type 18 E6 polypeptide in cells derived from human cervical carcinomas

L Banks et al. J Gen Virol. 1987 May.

Abstract

We recently reported the expression of human papillomavirus type 18 (HPV-18) E6 protein in bacteria and the production of anti-E6 polyclonal antibodies. This work has now been extended with the production of a panel of monoclonal antibodies against the HPV-18 E6 protein. These antibodies demonstrate that there is little antigenic conservation in the E6 protein between HPV-16 and HPV-18, with only one antibody recognizing a cross-reactive epitope. We have used both the monoclonal and the polyclonal antibodies to look for E6 expression in a number of HPV DNA-containing cell lines. These reagents specifically detected a 16.5K mol. wt. polypeptide in cells derived from a human cervical carcinoma.

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