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. 1987 May;61(5):1712-6.
doi: 10.1128/JVI.61.5.1712-1716.1987.

Expression of polyomavirus large T antigen by using a baculovirus vector

W C Rice, H E Lorimer, C Prives, L K Miller

Expression of polyomavirus large T antigen by using a baculovirus vector

W C Rice et al. J Virol. 1987 May.

Abstract

A gene encoding the large T antigen of polyomavirus was inserted into the baculovirus Autographa californica nuclear polyhedrosis virus so that gene expression was under the control of the strong, very late polyhedrin gene promoter. Significantly more large T antigen was produced in recombinant virus-infected insect cells than was observed in polyomavirus-transformed mouse cells. The insect-derived T antigen exhibited polyomavirus origin-specific DNA binding. The baculovirus expression system provides a convenient source of T antigen for in vitro studies.

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References

    1. EMBO J. 1986 Jun;5(6):1359-65 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Nature. 1985 Jun 13-19;315(6020):592-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1941-5 - PubMed
    1. J Virol. 1985 May;54(2):532-45 - PubMed

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