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. 2019 Jan 15;199(2):240-243.
doi: 10.1164/rccm.201807-1279LE.

IL-5 Levels in Nasosorption and Sputosorption Correlate with Sputum Eosinophilia in Allergic Asthma

Jose T Melo Jr  1 , Tanushree Tunstall  2 , Marcia M M Pizzichini  1 , Rosemeri Maurici  1 , Cristiane C Rocha  1 , Felipe Dal-Pizzol  1 , Jessica Gonçalves  1 , Trevor T Hansel  2 , Ryan S Thwaites  2 , Emilio Pizzichini  1
Affiliations

IL-5 Levels in Nasosorption and Sputosorption Correlate with Sputum Eosinophilia in Allergic Asthma

Jose T Melo Jr et al. Am J Respir Crit Care Med. .
No abstract available

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Figures

Figure 1.
Figure 1.
Sputosorption and nasosorption reveal IL-5 to be selectively increased in the asthmatic airway. (A) Sputum eosinophil levels (left) and nasosorption and sputosorption IL-5 levels (right) in healthy control patients (HCs; n = 17) and patients with asthma (n = 29). Bars represent median and interquartile range. Between-group comparisons by Mann-Whitney U test; ***P < 0.001. (B) Heat map of nasosorption and sputosorption levels of a panel of cytokines and chemokines (IL-5, IL-10, IL-13, tumor necrosis factor-α, CXCL8/IL-8, and CCL11) in HCs and allergic asthmatics (AAs). Mediator levels were normalized (Z scores) and were hierarchically clustered. Levels of mediators between HCs and AAs were compared using Mann-Whitney test and corresponding P values summarized: *P < 0.05; ***P < 0.001. (C) Spearman rank correlations between matched sputosorption and nasosorption samples in HCs and AAs: **P < 0.01; ***P < 0.001.
Figure 2.
Figure 2.
Airway IL-5 levels are closely associated with eosinophilic asthma. (A) Correlation matrix of nasosorption and sputosorption ILs and sputum eosinophils, neutrophils, and macrophages in patients with asthma (n = 29); blank squares denote insignificant correlations; color denotes Spearman R value. (B) Nasosorption IL-5 levels in allergic asthmatics stratified on a 3% cut-off for defining sputum eosinophilia (Eos). (C) Sputosorption IL-5 levels in allergic asthmatics with the 3% threshold for defining sputum eosinophilia. (<3% Eos: n = 13, >3%Eos: n = 16). *P = 0.037; **P = 0.001.
See this image and copyright information in PMC

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