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. 2018 Oct 17;16(10):389.
doi: 10.3390/md16100389.

Structure and Anti-Inflammatory Activity of a New Unusual Fucosylated Chondroitin Sulfate from Cucumaria djakonovi

Nadezhda E Ustyuzhanina  1 , Maria I Bilan  2 , Elena G Panina  3 , Nadezhda P Sanamyan  4 , Andrey S Dmitrenok  5 , Eugenia A Tsvetkova  6 , Natalia A Ushakova  7 , Alexander S Shashkov  8 , Nikolay E Nifantiev  9 , Anatolii I Usov  10
Affiliations

Structure and Anti-Inflammatory Activity of a New Unusual Fucosylated Chondroitin Sulfate from Cucumaria djakonovi

Nadezhda E Ustyuzhanina et al. Mar Drugs. .

Abstract

Fucosylated chondroitin sulfate CD was isolated from the sea cucumber Cucumaria djakonovi collected from the Avachinsky Gulf of the eastern coast of Kamchatka. Structural characterization of CD was performed using a series of non-destructive NMR spectroscopic procedures. The polysaccharide was shown to contain a chondroitin core [→3)-β-d-GalNAc-(1→4)-β-d-GlcA-(1→]n where about 60% of GlcA residues were 3-O-fucosylated, while another part of GlcA units did not contain any substituents. The presence of unsubstituted both at O-2 and O-3 glucuronic acid residues in a structure of holothurian chondroitin sulfate is unusual and has not been reported previously. Three different fucosyl branches Fucp2S4S, Fucp3S4S and Fucp4S were found in the ratio of 2:1:1. The GalNAc units were mono- or disulfated at positions 4 and 6. Anti-inflammatory activity of CD was assessed on a model of acute peritoneal inflammation in rats. About 45% inhibition was found for CD, while a structurally related linear chondroitin sulfate SS from cartilage of the fish Salmo salar demonstrated only 31% inhibition, indicating that the presence of sulfated fucosyl branches is essential for anti-inflammatory effect of chondroitin sulfates of marine origin.

Keywords: Cucumaria djakonovi; anti-inflammatory activity; fucosylated chondroitin sulfate; sea cucumber; structure.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1
Figure 1
Electrophoresis in polyacrylamide gel. Hep—heparin (Sigma), Enox—enoxaparin (Clexane®, Sanofi), SS—chondroitin sulfate from S. salar, CD—fucosylated chondroitin sulfate from C. djakonovi.
Figure 2
Figure 2
The 13C NMR spectrum of fucosylated chondroitin sulfate CD.
Figure 3
Figure 3
The 1H NMR spectrum of fucosylated chondroitin sulfate CD.
Figure 4
Figure 4
Repeating branched (I) and linear (II) blocks of fucosylated chondroitin sulfate CD (units A–K) and disaccharide components of chondroitin sulfate SS (units G, G’, J, K). Unit A bears Fuc2S4S (D), whereas unit A’ bears Fuc3S4S (E) or Fuc4S (F). Unit G is linked to GalNAc4S6S (H) or GalNAc4S (J), whereas unit G’ is linked to GalNAc6S (K).
Figure 5
Figure 5
The NMR spectra of fucosylated chondroitin sulfate CD: 1H-13C HSQC (A), 1H-1H COSY (B), ROESY (C). Correlation peaks are marked in the spectra by letter designation of units used in Figure 4 and Table 1.
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References

    1. Wu M., Xu L., Zhao L., Xiao C., Gao N., Luo L., Yang L., Li Z., Chen L., Zhao J. Structural analysis and anticoagulant activities of the novel sulfated fucan possessing a regular well-defined repeating unit from sea cucumber. Mar. Drugs. 2015;13:2063–2084. doi: 10.3390/md13042063. - DOI - PMC - PubMed
    1. Yu L., Xue C., Chang Y., Hu Y., Xu X., Ge L., Liu G. Structure and rheological characteristics of fucoidan from sea cucumber Apostichopus japonicus. Food Chem. 2015;180:71–76. doi: 10.1016/j.foodchem.2015.02.034. - DOI - PubMed
    1. Hu Y., Li S., Li J., Ye X., Ding T., Liu D., Chen J., Ge Z., Chen S. Identification of a highly sulfated fucoidan from sea cucumber Pearsonothuria graeffei with well-repeated tetrasaccharide units. Carbohydr. Polym. 2015;134:808–816. doi: 10.1016/j.carbpol.2015.06.088. - DOI - PubMed
    1. Chang Y., Hu Y., Yu L., McClements D.J., Xu X., Liu G., Xue C. Primary structure and chain conformation of fucoidan extracted from sea cucumber Holothuria tubulosa. Carbohyd. Polym. 2016;136:1091–1097. doi: 10.1016/j.carbpol.2015.10.016. - DOI - PubMed
    1. Li S., Li J., Zhi Z., Wei C., Wang W., Ding T., Ye X., Hu Y., Linhardt R.J., Chen S. Macromolecular properties and hypolipidemic effects of four sulfated polysaccharides from sea cucumbers. Carbohydr. Polym. 2017;173:330–337. doi: 10.1016/j.carbpol.2017.05.063. - DOI - PubMed

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