Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 May;84(9):2803-7.
doi: 10.1073/pnas.84.9.2803.

Tissue-specific expression of the human type II collagen gene in mice

R H Lovell-Badge, A Bygrave, A Bradley, E Robertson, R Tilly, K S Cheah

Tissue-specific expression of the human type II collagen gene in mice

R H Lovell-Badge et al. Proc Natl Acad Sci U S A. 1987 May.

Abstract

Type II collagen is crucial to the development of form in vertebrates as it is the major protein of cartilage. To study the factors regulating its expression we introduced a cosmid containing the human type II collagen gene, including 4.5 kilobases of 5' and 2.2 kilobases of 3' flanking DNA, into embryonic stem cells in vitro. The transformed cells contribute to all tissues in chimeric mice allowing the expression of the exogenous gene to be studied in vivo. Human type II collagen mRNA is restricted to tissues showing transcription from the endogenous gene and human type II collagen is found in extracellular matrix surrounding chondrocytes in cartilage. The results indicate that the cis-acting requirements for correct temporal and spatial regulation of the gene are contained within the introduced DNA.

PubMed Disclaimer

References

    1. Dev Biol. 1976 Oct 15;53(2):153-70 - PubMed
    1. Nature. 1981 Jul 9;292(5819):154-6 - PubMed
    1. J Embryol Exp Morphol. 1981 Oct;65:73-87 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7634-8 - PubMed
    1. Mol Cell Biol. 1982 Feb;2(2):190-8 - PubMed

Publication types