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. 1987 Mar;161(2):559-66.
doi: 10.1016/0003-2697(87)90489-1.

Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions

W F Beyer Jr, I Fridovich

Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions

W F Beyer Jr et al. Anal Biochem. 1987 Mar.

Abstract

Most assays for superoxide dismutase depend upon competition between the enzyme and some indicating scavenger for O-2. We have investigated the effects of experimental variables on assays based upon the use of either ferricytochrome c or nitro blue tetrazolium. Our results should help investigators to avoid the numerous potential pitfalls which necessarily surround these assay methods.

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