doi: 10.1016/0003-2697(87)90489-1.
Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions
- PMID: 3034103
- DOI: 10.1016/0003-2697(87)90489-1
Item in Clipboard
Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions
Anal Biochem.
1987 Mar.
Abstract
Most assays for superoxide dismutase depend upon competition between the enzyme and some indicating scavenger for O-2. We have investigated the effects of experimental variables on assays based upon the use of either ferricytochrome c or nitro blue tetrazolium. Our results should help investigators to avoid the numerous potential pitfalls which necessarily surround these assay methods.
Similar articles
-
Spectrophotometric assay for superoxide dismutase based on tetrazolium salt 3'--1--(phenylamino)-carbonyl--3, 4-tetrazolium]-bis(4-methoxy-6-nitro)benzenesulfonic acid hydrate reduction by xanthine-xanthine oxidase.Anal Biochem. 1997 Sep 5;251(2):206-9. doi: 10.1006/abio.1997.2273. Anal Biochem. 1997. PMID: 9299017
-
Enzymatic and non-enzymatic assay of superoxide dismutase.Biochimie. 1975;57(5):657-60. doi: 10.1016/s0300-9084(75)80147-7. Biochimie. 1975. PMID: 171001
-
Superoxide dismutase assay using alkaline dimethylsulfoxide as superoxide anion-generating system.Anal Biochem. 1983 Dec;135(2):280-7. doi: 10.1016/0003-2697(83)90684-x. Anal Biochem. 1983. PMID: 6318599
-
A critical reevaluation of some assay methods for superoxide dismutase activity.Free Radic Biol Med. 1988;4(5):295-303. doi: 10.1016/0891-5849(88)90050-0. Free Radic Biol Med. 1988. PMID: 2834277
-
Superoxide dismutases.Adv Enzymol Relat Areas Mol Biol. 1974;41(0):35-97. doi: 10.1002/9780470122860.ch2. Adv Enzymol Relat Areas Mol Biol. 1974. PMID: 4371571 Review. No abstract available.
Cited by
-
Impact of Biochar Application at Water Shortage on Biochemical and Physiological Processes in Medicago ciliaris.Plants (Basel). 2022 Sep 15;11(18):2411. doi: 10.3390/plants11182411. Plants (Basel). 2022. PMID: 36145812 Free PMC article.
-
Responses to Increased Salinity and Severe Drought in the Eastern Iberian Endemic Species Thalictrum maritimum (Ranunculaceae), Threatened by Climate Change.Plants (Basel). 2020 Sep 23;9(10):1251. doi: 10.3390/plants9101251. Plants (Basel). 2020. PMID: 32977553 Free PMC article.
-
Nitric Oxide Enhances Photosynthetic Nitrogen and Sulfur-Use Efficiency and Activity of Ascorbate-Glutathione Cycle to Reduce High Temperature Stress-Induced Oxidative Stress in Rice (Oryza sativa L.) Plants.Biomolecules. 2021 Feb 18;11(2):305. doi: 10.3390/biom11020305. Biomolecules. 2021. PMID: 33670537 Free PMC article.
-
Arbuscular mycorrhizal fungi enhance drought resistance in Bombax ceiba by regulating SOD family genes.PeerJ. 2024 Aug 7;12:e17849. doi: 10.7717/peerj.17849. eCollection 2024. PeerJ. 2024. PMID: 39131625 Free PMC article.
-
Responses to drought induced oxidative stress in five finger millet varieties differing in their geographical distribution.Physiol Mol Biol Plants. 2011 Oct;17(4):347-53. doi: 10.1007/s12298-011-0084-4. Epub 2011 Sep 23. Physiol Mol Biol Plants. 2011. PMID: 23573028 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
