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Comparative Study
. 1987 Jan;146(4):338-40.
doi: 10.1007/BF00410932.

Methylation sensitivity of the restriction enzymes FnuDII and AccII

M L Gaido, J S Strobl
Comparative Study

Methylation sensitivity of the restriction enzymes FnuDII and AccII

M L Gaido et al. Arch Microbiol. 1987 Jan.

Abstract

The restriction enzymes FnuDII and AccII are isoschizomers of the DNA sequence 5'-CGCG-3'. We have determined that 5-methylcytidine at either cytidine position in this recognition sequence inhibits DNA cleavage by FnuDII and AccII. A third isoschizomer, ThaI was previously shown to exhibit an identical methylation sensitivity. It is remarkable that 3 restriction enzymes derived from diverse microbiological sources exhibit this identical methylation sensitivity.

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