doi: 10.1007/BF00410941.
Construction of a transposon containing a gene for polygalacturonate trans-eliminase from Klebsiella oxytoca
- PMID: 3034186
- DOI: 10.1007/BF00410941
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Construction of a transposon containing a gene for polygalacturonate trans-eliminase from Klebsiella oxytoca
Arch Microbiol.
1987 Jan.
Abstract
A DNA fragment containing a Klebsiella oxytoca gene for polygalacturonate trans-eliminase was cloned into the kanamycin resistance transposon Tn5. This new transposon, designated Tn5-Pga+, had a transposition frequency of 1 X 10(-6). The broad host range plasmid pR751::Tn5-Pga+ was conjugally transferred to a variety of genetic backgrounds. The ability to degrade polygalacturonate was expressed in Aeromonas hydrophila, Alcaligenes eutrophus, Azotomonas insolita, Escherichia coli, Pseudomonas putida and Rhodopseudomonas sphaeroides, but not in Zymomonas mobilis.
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