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. 1987 Jan;146(4):390-5.
doi: 10.1007/BF00410941.

Construction of a transposon containing a gene for polygalacturonate trans-eliminase from Klebsiella oxytoca

M J Walker, J M Pemberton

Construction of a transposon containing a gene for polygalacturonate trans-eliminase from Klebsiella oxytoca

M J Walker et al. Arch Microbiol. 1987 Jan.

Abstract

A DNA fragment containing a Klebsiella oxytoca gene for polygalacturonate trans-eliminase was cloned into the kanamycin resistance transposon Tn5. This new transposon, designated Tn5-Pga+, had a transposition frequency of 1 X 10(-6). The broad host range plasmid pR751::Tn5-Pga+ was conjugally transferred to a variety of genetic backgrounds. The ability to degrade polygalacturonate was expressed in Aeromonas hydrophila, Alcaligenes eutrophus, Azotomonas insolita, Escherichia coli, Pseudomonas putida and Rhodopseudomonas sphaeroides, but not in Zymomonas mobilis.

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