Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Sep-Oct;34(5):1070-1075.
doi: 10.12669/pjms.345.15823.

Does extracorporeal shockwave lithotripsy therapy affect thiol-disulfide homeostasis?

Aliseydi Bozkurt  1 , Cuma Mertoglu  2 , Mehmet Karabakan  3 , Gulsah Siranli  4 , Emine Feyza Yurt  5 , Ozcan Erel  6
Affiliations

Does extracorporeal shockwave lithotripsy therapy affect thiol-disulfide homeostasis?

Aliseydi Bozkurt et al. Pak J Med Sci. 2018 Sep-Oct.

Abstract

Objective: Extracorporeal Shockwave Lithotripsy (ESWL) is a non-invasive method that is effective at crushing stones in the upper urinary tract. Disturbance of the thiol/disulfide homeostasis, in favor of the disulfide, has been shown to be involved in the disease pathogenesis.

Methods: A total of 36 individuals that underwent ESWL had blood samples collected before ESWL (0hrs), 6hrs, and one week after the ESWL. Sera native and total as wells as disulfide amount was measured using an automated method sodium borohydrate (NaBH4) reduction. In addition, Ischemia Modified Albumin (IMA) levels were measured using colorimetric assay method.

Results: Native thiol level was reduced at the 6th hour following ESWL compared to baseline. While the ratios of disulfide level, Disulfide/Total Thiol (DTT), Disulfide/Native Thiol (DNT) and IMA level were increased at the 6th hour following ESWL compared to baseline, they were found to be similar with their baseline values at the end of 1st week. Total thiol and native /total thiol did not show any significant change.

Conclusions: ESWL treatment disrupts thiol/disulfide homeostasis and the structure of albumin at the acute term. Therefore, it increases protein oxidation and leads to increased oxidative stress. However, this state is transient and returns to normal within the proceeding days.

Keywords: Extracorporeal shockwave lithotripsy; Ischaemia modified albumin; Kidney stone; Oxidative stress; Thiol/disulfide.

PubMed Disclaimer

Figures

Fig.1
Fig.1
The change in mean native thiol levels measured before ESWL (native thiol 1) after 6 hour (native thiol 2), and 7th day (native thiol 3) from ESWL.
Fig.2
Fig.2
The change in mean disulfide levels measured before ESWL (disulfide 1), after 6 hour (disulfide 2), and 7th day (disulfide 3) from ESWL.
Fig.3
Fig.3
The change in median ischemia-modified albumin levels measured before ESWL (IMA 1), after 6 hour (IMA 2), and 7th day (IMA 3) from ESWL.
See this image and copyright information in PMC

References

    1. Turk C, Petrik A, Sarica K, Seitz C, Skolarikos A, Straub M, et al. EAU Guidelines on Interventional Treatment for Urolithiasis. Eur Urol. 2016;69(3):475–482. doi:10.1016/j.eururo.2015.07.041. - PubMed
    1. Miller NL, Lingeman JE. Management of kidney stones. Brit Med J. 2007;334:468–472. doi:10.1136/bmj.39113.480185.80. - PMC - PubMed
    1. Gecit I, Kavak S, Meral I, Pirincci N, Gunes M, Demir H, et al. Effects of shock waves on oxidative stress, antioxidant enzyme and element levels in kidney of rats. Biol Trace Elem Res. 2011;144(1–3):1069–1076. doi:10.1007/s12011-011-9124-8. - PubMed
    1. Sen CK, Packer L. Thiol homeostasis and supplements in physical exercise. Am J Clin Nutri. 2000 - PubMed
    1. Cremers CM, Jakob U. Oxidant sensing by reversible disulfide bond formation. J Biologi Chem. 2013;288:26489–26496. doi:10.1074/jbc.R113.462929. - PMC - PubMed

LinkOut - more resources