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. 1987 Mar;6(3):815-21.
doi: 10.1002/j.1460-2075.1987.tb04824.x.

Contacts between gamma delta resolvase and the gamma delta res site

E Falvey, N D Grindley

Contacts between gamma delta resolvase and the gamma delta res site

E Falvey et al. EMBO J. 1987 Mar.

Abstract

We have investigated the interaction between resolvase and the res site of the transposon gamma delta by methylation and ethylation interference experiments. We have examined the effect of these DNA modifications both on binding and resolution in vitro. Major groove methylations within a 9 bp sequence that borders each site inhibit binding of resolvase to that site. Ethylation of certain phosphates within, and adjacent to, this border sequence inhibits binding. Together, these interference points define a contact region, present at all three res sites. In vitro resolution is inhibited only by modifications within site I. Inhibition of resolution by methylation of adenines at the center of site I suggests that minor groove contacts near the crossover may be required for resolution activity.

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References

    1. Cell. 1980 Jan;19(1):1-11 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Jan;77(1):122-6 - PubMed
    1. Methods Enzymol. 1980;65(1):499-560 - PubMed
    1. Nucleic Acids Res. 1980 Feb 25;8(4):759-66 - PubMed
    1. Cell. 1981 Sep;25(3):713-9 - PubMed

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