Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986;50(1-3):101-9.
doi: 10.1016/0378-1119(86)90314-8.

Transposition studies of mini-Mu plasmids constructed from the chemically synthesized ends of bacteriophage Mu

T A Patterson, D L Court, G Dubuc, J J Michniewicz, J Goodchild, A I Bukhari, S A Narang

Transposition studies of mini-Mu plasmids constructed from the chemically synthesized ends of bacteriophage Mu

T A Patterson et al. Gene. 1986.

Abstract

We describe below the chemical synthesis of the right and left ends of bacteriophage Mu and characterize the activity of these synthetic ends in mini-Mu transposition. Mini-Mu plasmids were constructed which carry the synthetic Mu ends together with the Mu A and B genes under control of the bacteriophage lambda pL promoter. Derepression of pL leads to a high frequency of mini-Mu transposition (5.6 X 10(-2) which is dependent on the presence of the Mu ends and the Mu A and B proteins. Five deletion mutants in the Mu ends were tested in the mini-Mu transposition system and their effects on transposition are described.

PubMed Disclaimer

Publication types

Associated data

LinkOut - more resources