Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions
- PMID: 3034856
- PMCID: PMC212082
- DOI: 10.1128/jb.169.6.2424-2431.1987
Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions
Abstract
We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous