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Comparative Study
. 1987 Jun;169(6):2424-31.
doi: 10.1128/jb.169.6.2424-2431.1987.

Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions

Comparative Study

Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions

C W Ronson et al. J Bacteriol. 1987 Jun.

Abstract

We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.

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References

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