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. 1987 Jun;169(6):2639-42.
doi: 10.1128/jb.169.6.2639-2642.1987.

Identification, mapping, and cloning of the gene encoding cyanase in Escherichia coli K-12

Y C Sung, D Parsell, P M Anderson, J A Fuchs

Identification, mapping, and cloning of the gene encoding cyanase in Escherichia coli K-12

Y C Sung et al. J Bacteriol. 1987 Jun.

Abstract

The gene in Escherichia coli for cyanase, designated cynS, was localized to a BglII restriction site approximately 1.7 kilobases from the lacA end of the lac operon. The gene was cloned into the pUC13 vector. Maxicell analysis of plasmid-encoded proteins confirmed that the BglII site is in the region encoding the structural gene for cyanase. Cyanase-deficient strains had increased sensitivity to cyanate and were not able to use cyanate as a nitrogen source.

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