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. 2018 Oct 22;8(1):15593.
doi: 10.1038/s41598-018-32756-w.

A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au

Mohadeseh Zarei-Ghobadi  1   2 , Sayed-Hamidreza Mozhgani  3   4 , Fariba Dashtestani  1 , Amir Yadegari  5 , Fatemeh Hakimian  1 , Mehdi Norouzi  2   4 , Hedayatollah Ghourchian  6
Affiliations

A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au

Mohadeseh Zarei-Ghobadi et al. Sci Rep. .

Abstract

Carbon dots and Fe3O4@Au were synthesized to develop a new biosensor to detect DNA target. We investigated the photoluminescence property of carbon dots (CDs) in the presence of Fe3O4-capped Au (Fe3O4@Au). Firstly, we designed two dedicated probes for unique long sequence region of human T-lymphotropic virus type 1 genome. One of the probes was covalently bound to the CDs. In the absence of target, CDs-probe was adsorbed on the surface of Fe3O4@Au through two possible mechanisms, leading to quenching the fluorescence emission of CDs. The fluorescence emission of CDs was recovered in the presence of target since double-stranded DNA cannot adsorb on the Fe3O4@Au. Also, Fe3O4@Au can adsorb the unhybridized oligonucleotides and improves the accuracy of detection. The specificity of the proposed biosensor was confirmed by BLAST search and assessed by exposing the biosensor to other virus targets. The experimental detection limit of the biosensor was below 10 nM with linear range from 10 to 320 nM.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Schematic illustration of the detection steps of DNA target.
Figure 2
Figure 2
The FT-IR spectra of (A) Fe3O4@Au and (B) CDs.
Figure 3
Figure 3
(A)TEM image and (B) DLS analysis of Fe3O4@Au; (C) TEM image and (D) DLS analysis of CDs.
Figure 4
Figure 4
(A) XPS survey spectra of CDs, (B) C1s high-resolution XPS spectra, (C) O1s high resolution XPS spectra and (D) N1s high-resolution XPS spectra of CDs.
Figure 5
Figure 5
(A) The fluorescence emission spectra of CDs before and after conjugation with probe A. (B) The fluorescence emission spectra of CDs and UV-Vis absorption spectra of Fe3O4@Au solution.
Figure 6
Figure 6
The fluorescence emission spectra of CDs-probe A before (control) and after adsorption on the Fe3O4@Au surface.
Figure 7
Figure 7
The fluorescence spectra of the CDs-probe A as a function of target concentrations. The inset shows the linear range of the biosensor response.
Figure 8
Figure 8
Specificity of the biosensor for human T-lymphotropic virus-1 (HTLV-1) rather than human immunodeficiency virus (HIV) and hepatitis B virus (HBV). The concentration of all samples was 544 nM.
See this image and copyright information in PMC

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