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Meta-Analysis
. 2018 Nov 23;38(6):BSR20181676.
doi: 10.1042/BSR20181676. Print 2018 Dec 21.

Association of the WNT3 polymorphisms and non-syndromic cleft lip with or without cleft palate: evidence from a meta-analysis

Bing-Qian Wang  1 , Shu-Tao Gao  2 , Kun Chen  3 , Zhu-Qiu Xu  4 , Jia-Ming Sun  1 , Yun Xia  5 , Zheng-Tao Lv  6
Affiliations
Meta-Analysis

Association of the WNT3 polymorphisms and non-syndromic cleft lip with or without cleft palate: evidence from a meta-analysis

Bing-Qian Wang et al. Biosci Rep. .

Abstract

Objective: This meta-analysis was conducted with the aim of investigating the association between WNT3 gene polymorphisms and non-syndromic cleft lip (CL) with or without cleft palate (NSCL/P) predisposition.

Methods: A comprehensive literature search was performed in six online databases including PubMed, Embase, ISI Web of Science, CENTRAL, CNKI, and Wanfang from inception up to June 2018 without language restriction. Pooled odds ratios (ORs) and corresponding 95% confidence intervals (95%CIs) were calculated under allele model of inheritance to indicate the association between WNT3 polymorphisms and NSCL/P. Risk of bias was assessed through the Newcastle-Ottawa scale (NOS). Predetermined stratified and sensitivity analyses were performed using the RevMan 5.3 software, publication bias were evaluated by Egger's and Begg's tests.

Results: Seven case-control studies comprising 1617 NSCL/P patients and 2143 healthy controls were identified and included in the present study, a total of eight loci were investigated in the present study: rs3809857 was significantly associated with NSCL/P vulnerability (G compared with T, OR = 1.34, 95%CI: 1.15-1.56, P=0.0001), a significant association between rs9890413 polymorphism and NSCL/P susceptibility (A compared with G, OR = 1.25, 95%CI: 1.06-1.47, P=0.007) was detected as well. Since only few studies reported detailed data about the association between rs142167, rs7207916, rs199498, rs111769, rs12452064, rs11653738, and NSCL/P risk, these results were not combined using meta-analysis.

Conclusion: Based on the findings of our current study, the rs3809857 and rs9890413 polymorphisms of WNT3 appeared to be associated with NSCL/P. Limited evidence is found to support the association between other WNT3 polymorphisms and risk of NSCL/P.

Keywords: meta analysis; non-syndromic cleft lip with or without cleft palate; polymorphism; wnt3.

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Conflict of interest statement

The authors declare that there are no competing interests associated with the manuscript.

Figures

Figure 1
Figure 1. Flow diagram of literature search and screen
Figure 2
Figure 2. Forest plot of rs3809857 in WNT3 gene and risk of NSCL/P
Figure 3
Figure 3. Forest plot of rs9890413 in WNT3 gene and risk of NSCL/P
Figure 4
Figure 4. The leave-one-out sensitivity analysis of rs9890413 in WNT3 gene and risk of NSCL/P
Figure 5
Figure 5. Funnel plot of rs3809857 in WNT3 gene and risk of NSCL/P
Figure 6
Figure 6. Funnel plot of rs9890413 in WNT3 gene and risk of NSCL/P
Figure 7
Figure 7. QTL box plots of rs3809857 and rs9890413 in different types of human tissues based on the GTEx analysis.
eQTL box plots of rs3809857 and rs9890413 in human tissues: (A) rs3809857 and testis; (B) rs3809857 and pancreas; (C) rs3809857 and thyroid; (D) rs9890413 and subcutaneous adipose; (E) rs9890413 and tibial nerve; (F) rs9890413 and lung. Abbreviation: eQTL, expression Quantitative Trait Loci.
Figure 8
Figure 8. HaploReg view of rs3809857 and rs9890413 in WNT3 gene using HaploReg version 4.1.
HaploReg view of rs3809857 and rs9890413: (A) rs3809857; (B) rs9890413.
See this image and copyright information in PMC

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