Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Nov 20;32(5):424-433.
doi: 10.7555/JBR.32.20170065.

Cell-free plasma hypermethylated CASZ1, CDH13 and ING2 are promising biomarkers of esophageal cancer

Huan-Qiang Wang  1 , Cong-Ying Yang  2 , Si-Yuan Wang  3 , Tian Wang  1 , Jing-Ling Han  1 , Kai Wei  1 , Fu-Cun Liu  1 , Ji-da Xu  1 , Xian-Zhen Peng  1   4 , Jian-Ming Wang  5
Affiliations

Cell-free plasma hypermethylated CASZ1, CDH13 and ING2 are promising biomarkers of esophageal cancer

Huan-Qiang Wang et al. J Biomed Res. .

Abstract

Identifying sensitive and specific biomarkers for early detection of cancer is immensely imperative for early diagnosis and treatment and better clinical outcome of cancer patients. This study aimed to construct a specific DNA methylation pattern of cancer suppressor genes and explore the feasibility of applying cell-free DNA based methylation as a biomarker for early diagnosis of esophageal squamous cell carcinoma (ESCC). We recruited early stage ESCC patients from Yangzhong County, China. The Illumina Infinium 450K Methylation BeadChip was used to construct a genome-wide DNA methylation profile. Then, differentiated genes were selected for the validation study using the Sequenom MassARRAY platform. The frequency of methylation was compared between cancer tissues, matched cell-free DNAs and normal controls. The specific methylation profiles were constructed, and the sensitivity and specificity were calculated. Seven CG sites in three genes CASZ1, CDH13 and ING2 were significantly hypermethylated in ESCC as compared with normal controls. A significant correlation was found between the methylation of DNA extracted from cancer tissues and matched plasma cell-free DNA, either for individual CG site or for cumulative methylation analysis. The sensitivity and specificity reached 100% at an appropriate cut-point using these specific methylation biomarkers. This study revealed that aberrant DNA methylation is a promising biomarker for molecular diagnosis of esophageal cancer. Hypermethylation of CASZ1, CDH13 and ING2 detected in plasma cell-free DNA can be applied as a potential noninvasive biomarker for diagnosis of esophageal cancer.

PubMed Disclaimer

Figures

Fig.1
Fig.1
Flow chart of the study.
Fig.2
Fig.2
Scatter plot of individual CG site methylation in esophageal cancer tissue and plasma cell-free DNA.
Fig.3
Fig.3
Scatter plot of cumulative methylation detected in esophageal cancer tissue and plasma cell-free DNA.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Zhang Y. Epidemiology of esophageal cancer[J]. World J Gastroenterol, 2013, 19(34): 5598–5606 . - PMC - PubMed
    1. Pennathur A, Gibson MK, Jobe BA, et al. Oesophageal carcinoma[J]. Lancet, 2013, 381(9864): 400–412 . - PubMed
    1. Vizcaino AP, Moreno V, Lambert R, et al. Time trends incidence of both major histologic types of esophageal carcinomas in selected countries, 1973–1995[J]. Int J Cancer, 2002, 99(6): 860–868 . - PubMed
    1. Rutegård M, Charonis K, Lu Y, et al. Population-based esophageal cancer survival after resection without neoadjuvant therapy: an update[J]. Surgery, 2012, 152(5): 903–910 . - PubMed
    1. van Hagen P, Hulshof MC, van Lanschot JJ, et al. Preoperative chemoradiotherapy for esophageal or junctional cancer[J]. N Engl J Med, 2012, 366(22): 2074–2084 . - PubMed