Comparative study of the protective effects of chicken embryo amniotic fluid, vitamin C and coenzyme Q10 on cyclophosphamide-induced oxidative stress in mice ovaries

Abstract

Cyclophosphamide is a chemotherapy drug for the treatment of cancer. Chicken embryo amniotic fluid, vitamin C and coenzyme Q10 have anti-oxidant properties. Total of 70 adult female mice were selected and divided into seven groups. The first group that received 2 ml kg^-1 of inactivated amniotic fluid subcutaneously. The second group treated with 75 mg kg^-1of cyclophosphamide by intraperitoneal injection. Third to fifth groups received 1, 2, and 4 ml kg^-1 of chicken embryo amniotic fluid, respectively. The sixth group received vitamin C at a dose of 0.2 mg g^-1 of body weight by oral gavages. Seventh group received 10 mg kg^-1 coenzyme Q10 intraperitoneally. All cyclophosphamide treated groups (3-7) received 75 mg kg^-1 of cyclophosphamide intraperitoneal on day 22. The mice were euthanized on day 29 and ovarian tissue antioxidant enzymes including glutathione peroxidase, superoxide dismutase and catalase activities and malondialdehyde (MDA) were evaluated. Activities of above mentioned enzymes in treatment groups (3-7) was significantly higher than patient control group (2). The results also revealed that MDA levels were higher in the control group in comparison to other treatment groups. Therefore, it is concluded that the chick embryo amniotic fluid and coenzyme Q10 can compete with compounds like vitamin C in increasing the anti-oxidant level in ovarian tissue.

Keywords: Chicken embryo amniotic fluid; Coenzyme Q10; Cyclophosphamide; Oxidative stress; Vitamin C.

Fig. 1

Comparison of GPx, SOD and CAT content in seven groups: healthy control group (CH), patient control (CD), treatment with amniotic fluid at a dose of 1 mL kg^-1 (CAF1), treatment with amniotic fluid at a dose of 2 mL kg^-1 (CAF2), treatment with amniotic fluid at a dose of 4 mL kg^-1 (CAF4), vitamin C treatment (VIT C) and coenzyme Q10 treatment (COQ10). The significance level of above factors in seven groups is classified as a to g in relation to control group (p < 0.05).

Fig. 2

Comparison of MDA content in seven groups: healthy control group (CH), patient control (CD), treatment with amniotic fluid at a dose of 1 mL kg^-1 (CAF1), treatment with amniotic fluid at a dose of 2 mL kg^-1 (CAF2), treatment with amniotic fluid at a dose of 4 mL kg^-1 (CAF4), vitamin C treatment (VIT C) and coenzyme Q10 treatment (COQ10). The significance level of above factors in seven groups is classified as a to f in relation to control group (p < 0.05).

Fig. 3

Cyclophosphamide induced ovarian toxicity. This medication increased lipid peroxidation and decreased GPx, SOD and CAT activities. Antioxidant compounds like vitamin C, coenzyme Q10 and chicks' embryo amniotic fluid can increase antioxidant status in ovarian tissue and cause fertility improvement.