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Review
. 2018 Dec;31(6):709-717.
doi: 10.1097/WCO.0000000000000613.

Proteomic changes in traumatic brain injury: experimental approaches

James L Sowers  1 , Ping Wu  2 , Kangling Zhang  3 , Douglas S DeWitt  4 , Donald S Prough  4
Affiliations
Review

Proteomic changes in traumatic brain injury: experimental approaches

James L Sowers et al. Curr Opin Neurol. 2018 Dec.

Abstract

Purpose of review: The underlying mechanisms responsible for chronic and progressive neurological damage after traumatic brain injury (TBI) are poorly understood, and therefore, current treatment options are limited. Proteomics is an emerging methodology to study changes to the TBI proteome in both patients and experimental models.

Recent findings: Although experimentally complex, mass spectrometry-based proteomics approaches are converging on a set of common methods. However, these methods are being applied to an increasingly diverse range of experimental models and types of injury.

Summary: In this review, our aim is to briefly describe experimental TBI models and the underlying methods common to most proteomic approaches. We will then review a series of articles that have recently appeared in which these approaches have been applied to important TBI questions. We will summarize several recent experimental studies, and suggest how the results of these emerging studies might impact future research as well as patient treatment.

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References

    1. López-García I, Gerő D, Szczesny B, et al. Development of a stretch-induced neurotrauma model for medium-throughput screening in vitro: identification of rifampicin as a neuroprotectant. Br J Pharmacol 2018; 175:284–300. - PMC - PubMed

    2. This study showed that proteomics could be used to help screen for/repurpose drugs for TBI.

    1. Cheng SX, Xu Z-W, Yi T-L, et al. iTRAQ-based quantitative proteomics reveals the new evidence base for traumatic brain injury treated with targeted temperature management. Neurotherapeutics 2018; 15:216–232. - PMC - PubMed

    2. Cheng et al. determined that the use of proteomics and bioinformatics analysis could improve the efficacy of hypothermia treatment and be potentially used for identifying patients for which the therapy might be beneficial.

    1. Song H, Fang S, Gao J, et al. Quantitative proteomic study reveals up-regulation of cAMP signaling pathway-related proteins in mild traumatic brain injury. J Proteome Res 2018; 17:858–869. - PubMed

    2. This study identified proteins that could help distinguish between single and repetitive head injury.

    1. Mattugini N, Merl-Pham J, Petrozziello E, et al. Influence of white matter injury on gray matter reactive gliosis upon stab wound in the adult murine cerebral cortex. Glia 2018; 66:1644–1662. - PubMed

    2. Mattugini et al. determined how the proteome response could change so much based upon grey matter injury vs. grey and white matter injury.

    1. Frik J, Merl-Pham J, Plesnila N, et al. Cross-talk between monocyte invasion and astrocyte proliferation regulates scarring in brain injury. EMBO Rep 2018; 19: doi:10.15252/embr.201745294. - PMC - PubMed

    2. Frik et al. used proteomics and genetic mouse models to elucidate how monocytes contribute to glial scar formation.

Publication types