Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Jun;127(3):409-13.

Detection of cytomegalovirus infection by means of DNA isolated from paraffin-embedded tissues and dot hybridization

E Seto, T S Yen

Detection of cytomegalovirus infection by means of DNA isolated from paraffin-embedded tissues and dot hybridization

E Seto et al. Am J Pathol. 1987 Jun.

Abstract

A specific and rapid method for detecting the presence of cytomegalovirus (CMV) DNA in formalin- or mercuric chloride-fixed, paraffin-embedded tissue has been developed. With dot hybridization with a biotinylated CMV DNA probe, the presence of CMV infection can be readily detected even if only a minority of the cells in the tissue block are infected. The sensitivity is further increased by about two orders of magnitude if high specific-activity 32P-labeled probe is used. This method of detection is more sensitive than histologic diagnosis, appears to be roughly comparable to that by virus isolation in culture, and should be applicable to the detection of other infectious agents.

PubMed Disclaimer

References

    1. J Mol Biol. 1981 Sep 5;151(1):17-33 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6999-7003 - PubMed
    1. Virology. 1983 Apr 15;126(1):32-50 - PubMed
    1. Hum Pathol. 1984 May;15(5):430-9 - PubMed
    1. Cancer Res. 1986 Jun;46(6):2964-9 - PubMed

Publication types