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. 2019:1875:345-358.
doi: 10.1007/978-1-4939-8837-2_26.

Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-Infected Plants

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Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-Infected Plants

Marilia Almeida-Trapp et al. Methods Mol Biol. 2019.

Abstract

There is strong evidence that phytohormones such as abscisic acid, auxin, salicylic acid, and jasmonates might play a role in defense of the host plants during phytoplasma infections. However, these compounds are usually present at low concentration in complex matrixes, requiring a sensitive and selective method to analyze and quantify them. Here, we present a HPLC-MS/MS method to quantify phytohormones in different infected and noninfected plant tissues.

Keywords: Abscisic acid; Auxin; HPLC-MS; Jasmonates; Jasmonic acid; Mass spectrometry; OPDA; Phytohormones; Quantification; Salicylic acid.

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