Diadinoxanthin de-epoxidation as important factor in the short-term stabilization of diatom photosynthetic membranes exposed to different temperatures

Abstract

The importance of diadinoxanthin (Ddx) de-epoxidation in the short-term modulation of the temperature effect on photosynthetic membranes of the diatom Phaeodactylum tricornutum was demonstrated by electron paramagnetic resonance (EPR), Laurdan fluorescence spectroscopy, and high-performance liquid chromatography. The 5-SASL spin probe employed for the EPR measurements and Laurdan provided information about the membrane area close to the polar head groups of the membrane lipids, whereas with the 16-SASL spin probe, the hydrophobic core, where the fatty acid residues are located, was probed. The obtained results indicate that Ddx de-epoxidation induces a two component mechanism in the short-term regulation of the membrane fluidity of diatom thylakoids during changing temperatures. One component has been termed the "dynamic effect" and the second the "stable effect" of Ddx de-epoxidation. The "dynamic effect" includes changes of the membrane during the time course of de-epoxidation whereas the "stable effect" is based on the rigidifying properties of Dtx. The combination of both effects results in a temporary increase of the rigidity of both peripheral and internal parts of the membrane whereas the persistent increase of the rigidity of the hydrophobic core of the membrane is solely based on the "stable effect."

Keywords: EPR; Laurdan fluorescence spectroscopy; diadinoxanthin de-epoxidation; diatoms; photosynthetic membranes; short-term thermal adaptation.