Impacts of molybdenum-, nickel-, and lithium- oxide nanomaterials on soil activity and microbial community structure

Abstract

The nano forms of the metals molybdenum oxide (MoO₃), nickel oxide (NiO) and lithium oxide (Li₂O) are finding wide application in advanced technologies including batteries and fuel cells. We evaluated soil responses to nanoMoO₃, nanoNiO, and nanoLi₂O as some environmental release of the materials, either directly or following the land application of biosolids, is expected. Using Drummer soil (Fine-silty, mixed, superactive, mesic Typic Endoaquolls), we evaluated the impacts of the three nanometals on soil gas (N₂O, CH₄, and CO₂) emissions, enzyme activities (β-glucosidase and urease), and microbial community structure (bacterial, archaeal, and eukaryal) in a 60 day microcosms incubation. Soil treated with nanoLi₂O at 474 μg Li/g soil, released 3.45 times more CO₂ with respect to the control. Additionally, β-glucosidase activity was decreased while urease activity increased following nanoLi₂O treatment. While no clear patterns were observed for gas emissions in soils exposed to nanoMoO₃ and nanoNiO, we observed a temporary suppression of β-glucosidase activity in soil treated with either metal. All three domains of microbial community were affected by increasing metal concentrations. This is the first evaluation of soil responses to nanoMoO₃, nanoNiO, or nanoLi₂O.

Keywords: Archaea; Bacteria; Eukarya; Soil gas emissions; Urease; β-glucosidase.