Serologic detection of active infections with human herpes viruses (CMV, EBV, HSV, VZV): diagnostic potential of IgA class and IgG subclass-specific antibodies

Abstract

In 175 sera from healthy persons as well as those suffering from primary or secondary herpes virus infections/reactivations, serum antibodies were assessed by an indirect ELISA in the immunoglobulin classes A, G and M and the subclasses G1-4, using carrier-fixed antigens (CMV, VZV, HSV) and monoclonal tracer antibodies. In a similar way EBV-specific antibodies were tested by an indirect IFT. Only IgG1 antibodies were detectable in nearly all persons. Virus-specific IgA and IgG3 may support conventional serological methods (IgM, IgG) indicating recent infection/reactivation with VZV, EBV and possibly CMV. Furthermore, differentiation of primary and secondary CMV and VZV infection was possible in some cases, when IgG3 was detectable before IgG1 in subsequent blood specimens. Recurrent herpes lesions could not be diagnosed serologically.