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Randomized Controlled Trial
. 2018 Oct 26;22(1):279.
doi: 10.1186/s13054-018-2077-y.

The effect of polymyxin B hemoperfusion on modulation of human leukocyte antigen DR in severe sepsis patients

Nattachai Srisawat  1   2   3 , Somkanya Tungsanga  4 , Nuttha Lumlertgul  4   5 , Chalermchai Komaenthammasophon  4   5 , Sadudee Peerapornratana  4   5   6 , Nicha Thamrongsat  4   5 , Khajohn Tiranathanagul  4 , Kearkiat Praditpornsilpa  4 , Somchai Eiam-Ong  4 , Kriang Tungsanga  4 , John A Kellum  6
Affiliations
Randomized Controlled Trial

The effect of polymyxin B hemoperfusion on modulation of human leukocyte antigen DR in severe sepsis patients

Nattachai Srisawat et al. Crit Care. .

Abstract

Background: Recent randomized trials have not found that polymyxin B hemoperfusion (PMX-HP) improves outcomes for patients with sepsis. However, it remains unclear whether the therapy could provide benefit for highly selected patients. Monocyte human leukocyte antigen (mHLA-DR) expression, a critical step in the immune response, is decreased during sepsis and leads to worsening sepsis outcomes. One recent study found that PMX-HP increased mHLA-DR expression while another found that the treatment removed HLA-DR-positive cells.

Methods: We conducted a randomized controlled trial in patients with blood endotoxin activity assay (EAA) level ≥ 0.6. Patients in the PMX-HP group received a 2-h PMX-HP treatment plus standard treatment for 2 consecutive days. Patients in the non-PMX-HP group received only standard treatment. The primary outcome compared the groups on median change in mHLA-DR expression between day 3 and baseline. Secondary outcomes compared the groups on the mean or median change in CD11b expression, neutrophil chemotaxis, presepsin, cardiovascular Sequential Organ Failure Assessment (CVS SOFA) score, vasopressor dose, and EAA level between day 3 and baseline. We further compared the groups on mortality, ICU-free days, ventilator-free days, dialysis dependence status, renal recovery, serum creatinine, vasopressor-free days, and major adverse kidney events (MAKE 28), measured on day 28.

Results: Fifty-nine patients were randomized to PMX-HP (n = 29) and non-PMX-HP (n = 30) groups. At baseline, mHLA-DR expression, CD11b, neutrophil chemotaxis, and clinical parameters were comparable between groups. The median change in mHLA-DR expression between day 3 and baseline was higher in PMX-HP patients than in patients receiving standard therapy alone (P = 0.027). The mean change in CD11b between day 3 and baseline was significantly lower in the PMX-HP group than in the non-PMX-HP group (P = 0.002). There were no significant changes from baseline in neutrophil chemotaxis, presepsin, CVS SOFA scores, vasopressor doses, or EAA level between groups. On day 28 after enrollment, mortality, ICU-free days, ventilator-free days, dialysis dependence status, renal recovery, serum creatinine, vasopressor-free days, and MAKE 28 were comparable between groups.

Conclusion: PMX-HP improved mHLA-DR expression in severe sepsis patients. Future studies should examine the potential benefit of PMX-HP in patients with low mHLA-DR expression.

Trial registration: ClinicalTrials.gov, NCT02413541 . Registered on 3 March 2015.

Keywords: Immunoparalysis; Polymyxin B hemoperfusion; Severe sepsis/septic shock.

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Conflict of interest statement

Ethics approval and consent to participate

Ethics approval was obtained from the Faculty Ethical Committee (IRB No. 578/56). Written inform consent or agreement to participate was obtained from each patient or the patient’s surrogate.

Competing interests

JAK reports grant support and consulting fees from Baxter and NxStage. Toray Industries provided the polymyxin B cartridge and endotoxin activity assay kits for use in this study.

The company had no influence on the study design or analysis or on the comment of this article. None of the other authors have any disclosures.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Flow diagram of patient allocation. EAA endotoxin activity assay, ICU intensive care unit, PMX-HP polymyxin B hemoperfusion
Fig. 2
Fig. 2
Comparison of mHLA-DR expression, CD11b expression, and chemotaxis on neutrophil between PMX-HP group and non-PMX-HP group. D day, mHLA-DR monocytic human leukocyte antigen, PMX-HP polymyxin B hemoperfusion
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