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Review
. 2018 Sep 27;17(4):434-441.
doi: 10.1002/rmb2.12245. eCollection 2018 Oct.

Updating the markers for oocyte quality evaluation: intracellular temperature as a new index

Yumi Hoshino  1
Affiliations
Review

Updating the markers for oocyte quality evaluation: intracellular temperature as a new index

Yumi Hoshino. Reprod Med Biol. .

Abstract

Background: The developmental competence of an embryo is principally dictated by the oocyte. Usually, oocyte selection is based on morphological properties; however, all morphological criteria that are currently used for the grading and screening of oocytes are not able to eliminate the subjectivity. Despite recent studies of the molecular factors related to oocyte quality, it is technically difficult to develop an index based on these factors, and new indices that reflect intracellular conditions are necessary.

Methods: Morphological and molecular factors influencing developmental competence were comprehensively reviewed, and intracellular temperature was evaluated as a new marker of oocyte quality.

Main findings: The intracellular temperature of mature oocytes was high in fresh oocytes and decreased with time after polar body release. Under the same conditions, the intracellular temperature and its distribution differed among oocytes, suggesting that temperature represents the state of each oocyte.

Conclusion: Intracellular temperature is advantageous as an objective and quantitative indicator of oocyte quality. Further studies should evaluate the link between temperature and cellular phenomena to establish its use as an indicator of quality.

Keywords: assisted reproductive techniques; meiosis; oocyte; temperature.

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Figures

Figure 1
Figure 1
Schematic representation of intracellular temperature in matured oocytes. A, Fresh oocyte, and B, overmatured or aged oocyte. Fresh oocytes had high‐temperature regions localized around the cell membrane and around the spindle. Red and yellow spots indicate high temperature, and blue and green spots indicate low temperature
See this image and copyright information in PMC

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