3D Models of Pancreatic Ductal Adenocarcinoma via Tissue Engineering

Abstract

Cancer tissue engineering is an emerging multidisciplinary field aimed at growing cancerous cells onto porous biomaterial scaffolds and proper stimuli to ultimately reproduce 3D tumor tissue-like constructs in vitro. Unlike conventional 2D cell cultures and spheroids, these tissue models can reproduce cancer lesions very similar to those present in native tumor, and can be viable for some weeks, making it possible to study cancer biology phenomena and new therapies in a more reliable fashion than with conventional in vitro platforms. This chapter shows the preparation of a 3D model of pancreatic ductal adenocarcinoma (PDAC), including fabrication of a suitable scaffold, culture of PDAC cells on the scaffold, viability test, and histologic assessment.

Keywords: Cancer model; Emulsion; Gelatin; Histology; PANC cells; Pancreatic ductal adenocarcinoma; Poly(vinyl alcohol) (PVA); Scaffold; Sponge; Three-dimensional (3D); Tissue engineering.