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. 2018 Oct 26;32(9):1430-1438.
doi: 10.5713/ajas.18.0414. Print 2019 Sep.

Effects of coated cysteamine hydrochloride on muscle fiber characteristics and amino acid composition of finishing pigs

Miaomiao Bai  1   2 , Hongnan Liu  1   3   4 , Kang Xu  1   3   4 , Rong Yu  3 , Abimbola Oladele Oso  5 , Jinping Deng  2 , Yulong Yin  1   2   3   4
Affiliations

Effects of coated cysteamine hydrochloride on muscle fiber characteristics and amino acid composition of finishing pigs

Miaomiao Bai et al. Asian-Australas J Anim Sci. .

Abstract

Objective: This experiment was designed to determine the effects of coated cysteamine hydrochloride (CC) on muscle fiber characteristics, amino acid composition and transporters gene expression in the longissimus dorsi muscle (LDM) of finishing pigs.

Methods: Two hundred and sixteen Duroc/Landrace/Yorkshire cross-bred male finishing pigs were fed with a corn-soybean basal diet supplemented with 0, 70 and 140 mg/kg cysteamine. Each group contained eight replicates of nine pigs per replicate. After 29 days, one pig was randomly selected from each replicate and slaughtered. Blood and LDM samples were collected and analyzed.

Results: The results showed that supplemental dietary CC increased (P < 0.05) the muscle fiber density. And CC supplementation also up-regulated (P < 0.05) the expression of MyHC1 and MyHC2x mRNA levels, and down-regulated (P < 0.05) MyHC2b expression in the LDM. Additionally, supplemental dietary CC reduced (P < 0.05) the concentration of total cholesterol in the plasma and enhanced (P < 0.05) the concentrations of essential amino acid and total amino acid in the LDM. The relative expression levels of CAT2, b0,+AT, and y+LAT1 were up-regulated (P < 0.05) in the LDM when pigs were fed with the dietary CC of 70 mg/kg.

Conclusion: Cysteamine supplementation could increase fiber density and distribution of fiber types. It also improved the deposition of protein in the LDM by up-regulated the expression of amino acid transporters.

Keywords: Amino Acids; Coated Cysteamine Hydrochloride; Finishing Pigs; Muscle Fiber.

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Conflict of interest statement

CONFLICT OF INTEREST

We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript. Liu H, Xu K, Yin Y are employees of Hangzhou King Techina Technology Co., Ltd, and Yu R, Yin Y are employees of CICAPS.

Figures

Figure 1
Figure 1
Morphology of longissimus dorsi muscle (LDM) fiber (200×). (A) Morphology of LDM fiber in pigs fed a basal diet containing 0 (CON). (B) Morphology of LDM fiber in pigs fed a basal diet containing 70 mg/kg (CC70). (C) Morphology of LDM fiber in pigs fed a basal diet containing 140 mg/kg (CC140).
Figure 2
Figure 2
Expression levels of muscle fiber types of finishing pigs fed a basal diet containing 0 (CON), 70 (CC70) and 140 mg/kg (CC140) coated cysteamine hydrochloride, respectively. The figure indicates: (A) MyHC1; (B) MyHC2x; (C) MyHC2a; (D) MyHC2b. The error bars represents standard error. Mean values with unlike letters (a, b) were significantly different (p<0.05).
Figure 3
Figure 3
Effects of dietary coated cysteamine hydrochloride (CC) on the relative mRNA expression levels of ASC amino-acid transporter 2 (ASCT2), cationic amino acid transporter (CAT)-1, CAT2, L-type amino acid transporter (LAT)-2, y+LAT1, and b0,+ amino acid transporter (b0,+AT) in (A, B, C, D, E and F) the longissimus dorsi muscle of finishing pigs. The figure indicates which pigs were fed with a corn-soybean diet containing the CCH of 0 (CON), 70 (CC70), and 140 mg/kg (CC140), respectively. The error bars represents standard error. Mean values with unlike letters (a, b) were significantly different (p<0.05).
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