doi: 10.1002/j.1460-2075.1987.tb02391.x.
DNA recognition by a new family of type I restriction enzymes: a unique relationship between two different DNA specificities
- PMID: 3038525
- PMCID: PMC553956
- DOI: 10.1002/j.1460-2075.1987.tb02391.x
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DNA recognition by a new family of type I restriction enzymes: a unique relationship between two different DNA specificities
EMBO J.
1987 May.
Abstract
The DNA sequences recognized by the allelic type I restriction enzymes EcoR124 and EcoR124/3 were determined. EcoR124 recognizes 5'-GAA(N6)RTCG-3' and EcoR124/3 recognizes 5'-GAA(N7)RTCG-3'. These are typical of sequences recognized by type I recognition enzymes in that they consist of two specific domains separated by a non-specific spacer sequence. For these two enzymes, the specific sequences are identical but the length of the non-specific spacer is different. The specific domains of EcoR124/3 are thus 3.4 A further apart than those of EcoR124 and rotated with respect to each other through a further 36 degrees.
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