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. 2018 Oct 12:20:e00286.
doi: 10.1016/j.btre.2018.e00286. eCollection 2018 Dec.

A new functional biofilm biocatalyst for the simultaneous removal of dibenzothiophene and quinoline using Rhodococcus rhodochrous and curli amyloid overproducer mutants derived from Cobetia sp. strain MM1IDA2H-1

M Alejandro Dinamarca  1 , Johana Eyzaguirre  2 , Patricio Baeza  2 , Paulina Aballay  2 , Christian Canales  3 , Juan Ojeda  4
Affiliations

A new functional biofilm biocatalyst for the simultaneous removal of dibenzothiophene and quinoline using Rhodococcus rhodochrous and curli amyloid overproducer mutants derived from Cobetia sp. strain MM1IDA2H-1

M Alejandro Dinamarca et al. Biotechnol Rep (Amst). .

Erratum in

Abstract

Biocatalyst systems based on biofilms were developed to remove nitrogen and sulfur-containing heterocyclic hydrocarbons using Cobetia sp. strain MM1IDA2H-1 and Rhodococcus rhodochrous. The curli overproducers mutants CM1 and CM4 were derived from Cobetia sp. strain and used to build monostrain biofilms to remove quinoline; and together with R. rhodochrous to simultaneously remove quinoline and dibenzothiophene using mixed biofilms. The quinoline removal using biofilms were 96% and 97% using CM1 or CM4 curli overproducers respectively, whereas bacterial suspensions assays yielded 19% and 24% with the same strains. At the other hand, the simultaneous removal of quinoline and dibenzothiophene using mixed biofilms were respectively 50% and 58% using strains R. rhodochrous with CM1 and 75% and 50% using R. rhodochrous with CM4. Results show that biofilms were more efficient than bacterial suspension assays and that in mixed biofilms the shared surface area by two or more bacteria could affect the final yield.

Keywords: Adsorption; Dibenzothiophene; Functional biofilm; Quinoline; Simultaneous removal.

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Figures

Fig. 1
Fig. 1
Phenotypes for curli over-production in mutants derived from Cobetia sp. strains MM1IDA2H-1. Image shows colonies after 24 h of incubations growing in agar Congo Red with pink light phenotype of wild type (a and b) and the red phenotype described for curli production (c and d). The selected curli over producer mutants where named: CM1 and CM4.
Fig. 2
Fig. 2
Removal of QN in packed bed column using biofilms of genetically modified Cobetia sp. strains MM1IDA2H-1 adsorbed on the surface of SiO2 particles, CM4 (-●-) and CM1 (⋅⋅○⋅⋅). The control was the QN removal on a packed bed column of silica without biofilm (⋅⋅▲⋅⋅). Data show mean values for three independent assays. Error bars correspond to the standard deviation.
Fig. 3
Fig. 3
Simultaneous removal of DBT and QN in a packed bed column using a functional biofilm formed by R. rhodochrous (-■-) and CM4 (-●-) adsorbed on SiO2 (RCM4), and a functional biofilm formed by R. rhodochrous (-□-) and CM1 (-○-) adsorbed on SiO2 (RCM1). The control (⋅⋅▲⋅⋅) was removal of DBT and QN on a packed bed column of silica without biofilm. Data are mean values for three independent assays. Error bars correspond to the standard deviation.
See this image and copyright information in PMC

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