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. 1987 Apr;7(2):173-83.
doi: 10.1089/jir.1987.7.173.

Structure and expression in Escherichia coli of canine interferon-alpha genes

A Himmler, R Hauptmann, G R Adolf, P Swetly

Structure and expression in Escherichia coli of canine interferon-alpha genes

A Himmler et al. J Interferon Res. 1987 Apr.

Abstract

Using a human interferon-alpha (IFN-alpha) cDNA probe, several recombinant phages containing type I IFN genes were isolated from a canine genomic library. One of these phages contains two complete CaIFN-alpha genes with identical coding sequences, and a second one a slightly different IFN-alpha gene. The IFN-alpha protein sequences contain six cysteine residues as well as two or three potential N-glycosylation sites. Expression of mature CaIFN-alpha 1 in E. coli results in antiviral activity on dog cells. Genomic analysis using an equine IFN-omega probe and DNA sequencing suggests the deletion of IFN-omega genes from canine genome.

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