doi: 10.1073/pnas.84.16.5570.
Multiple repressor binding sites in the genome of bacteriophage P1
- PMID: 3039493
- PMCID: PMC298904
- DOI: 10.1073/pnas.84.16.5570
Item in Clipboard
Multiple repressor binding sites in the genome of bacteriophage P1
Proc Natl Acad Sci U S A.
1987 Aug.
Abstract
After digestion of bacteriophage P1 DNA with EcoRI in the presence of P1 repressor, 6 repressor binding sites were identified in 5 of 26 EcoRI fragments. Binding sites were localized by the decreased mobility of DNA fragment-repressor complexes during electrophoresis and by DNase protection ("footprinting") analysis. The repressor binding sites, or operators, comprise a 17-base-pair-long consensus sequence lacking symmetrical elements. Three operators can be related to known genes, whereas the function of the others is still unknown. The mutant P1 bac, rendering ban expression constitutive, is identified as an operator-constitutive mutation of the ban operon.
Similar articles
-
ban operon of bacteriophage P1. Mutational analysis of the c1 repressor-controlled operator.J Mol Biol. 1989 Jan 5;205(1):127-35. doi: 10.1016/0022-2836(89)90370-7. J Mol Biol. 1989. PMID: 2647997
-
The c1 repressor of bacteriophage P1. Isolation and characterization of the repressor protein.J Biol Chem. 1988 Jan 25;263(3):1391-7. J Biol Chem. 1988. PMID: 2826478
-
The ban operon of bacteriophage P1. Localization of the promoter controlled by P1 repressor.J Biol Chem. 1987 Dec 5;262(34):16575-9. J Biol Chem. 1987. PMID: 3680265
-
Protein-DNA complexes: the cost of recognition.Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7429-30. doi: 10.1073/pnas.90.16.7429. Proc Natl Acad Sci U S A. 1993. PMID: 8356038 Free PMC article. Review. No abstract available.
-
Immunity and repression in bacteriophages P1 and P7.Curr Top Microbiol Immunol. 1980;90:49-65. doi: 10.1007/978-3-642-67717-5_3. Curr Top Microbiol Immunol. 1980. PMID: 7000446 Review. No abstract available.
Cited by
-
The c1 repressor of bacteriophage P1 operator-repressor interaction of wild-type and mutant repressor proteins.Nucleic Acids Res. 1989 Oct 11;17(19):7681-92. doi: 10.1093/nar/17.19.7681. Nucleic Acids Res. 1989. PMID: 2678004 Free PMC article.
-
Purification and characterization of UL9, the herpes simplex virus type 1 origin-binding protein.J Virol. 1992 Jul;66(7):3986-95. doi: 10.1128/JVI.66.7.3986-3995.1992. J Virol. 1992. PMID: 1318393 Free PMC article.
-
Positive regulation of glutamate biosynthesis in Bacillus subtilis.J Bacteriol. 1989 Sep;171(9):4718-27. doi: 10.1128/jb.171.9.4718-4727.1989. J Bacteriol. 1989. PMID: 2548995 Free PMC article.
-
Controlled expression in Klebsiella pneumoniae and Shigella flexneri using a bacteriophage P1-derived C1-regulated promoter system.J Bacteriol. 2001 Dec;183(23):6947-50. doi: 10.1128/JB.183.23.6947-6950.2001. J Bacteriol. 2001. PMID: 11698385 Free PMC article.
-
The c1 genes of P1 and P7.Nucleic Acids Res. 1989 Oct 11;17(19):7671-80. doi: 10.1093/nar/17.19.7671. Nucleic Acids Res. 1989. PMID: 2678003 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
