Cultivation of bloodstream Trypanosoma brucei
Bull World Health Organ.
1977.
Abstract
Animal-infective forms of Trypanosoma brucei (Strain 427) were successfully propagated in HEPES-buffered RPMI 1640 medium in the presence of bovine fibroblast-like cells for over 310 days. The organisms grown in this system were morphologically identical to the long slender bloodstream forms, retained their infectivity for mammalian hosts, and displayed variant-antigen on their surface. Technical details for establishing such bloodstream form cultures are described in the present paper.
Similar articles
-
African trypanosomes: cultivation of animal-infective Trypanosoma brucei in vitro.Science. 1977 May 27;196(4293):992-4. doi: 10.1126/science.558652. Science. 1977. PMID: 558652
-
An effective axenic culture system for Trypanosoma brucei rhodesiense blood stream forms in vitro.Southeast Asian J Trop Med Public Health. 1990 Dec;21(4):564-7. Southeast Asian J Trop Med Public Health. 1990. PMID: 2098917
-
Continuous growth of bloodstream forms of Trypanosoma brucei brucei in an axenic culture system containing a low concentration of serum.Southeast Asian J Trop Med Public Health. 1998 Sep;29(3):591-5. Southeast Asian J Trop Med Public Health. 1998. PMID: 10437963
-
Growth of infective forms of Trypanosoma (T.) brucei on buffalo lung and Chinese hamster lung tissue culture cells.Acta Trop. 1978 Sep;35(3):201-7. Acta Trop. 1978. PMID: 31773
-
Antigenic variation during the developmental cycle of Trypanosoma brucei.J Protozool. 1984 Feb;31(1):41-7. J Protozool. 1984. PMID: 6204043 Review.
Cited by
-
A molecular mechanism for eflornithine resistance in African trypanosomes.PLoS Pathog. 2010 Nov 24;6(11):e1001204. doi: 10.1371/journal.ppat.1001204. PLoS Pathog. 2010. PMID: 21124824 Free PMC article.
-
Trypanosoma brucei: Metabolomics for analysis of cellular metabolism and drug discovery.Metabolomics. 2022 Mar 19;18(4):20. doi: 10.1007/s11306-022-01880-0. Metabolomics. 2022. PMID: 35305174 Review.
-
Untargeted metabolomics reveals a lack of synergy between nifurtimox and eflornithine against Trypanosoma brucei.PLoS Negl Trop Dis. 2012;6(5):e1618. doi: 10.1371/journal.pntd.0001618. Epub 2012 May 1. PLoS Negl Trop Dis. 2012. PMID: 22563508 Free PMC article.
-
The bloodstream form of Trypanosoma brucei displays non-canonical gluconeogenesis.PLoS Negl Trop Dis. 2024 Feb 23;18(2):e0012007. doi: 10.1371/journal.pntd.0012007. eCollection 2024 Feb. PLoS Negl Trop Dis. 2024. PMID: 38394337 Free PMC article.
-
Gene co-expression network analysis of Trypanosoma brucei in tsetse fly vector.Parasit Vectors. 2021 Jan 22;14(1):74. doi: 10.1186/s13071-021-04597-6. Parasit Vectors. 2021. PMID: 33482903 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources