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. 1977;55(2-3):405-9.

Cultivation of bloodstream Trypanosoma brucei

H Hirumi, J J Doyle, K Hirumi

Cultivation of bloodstream Trypanosoma brucei

H Hirumi et al. Bull World Health Organ. 1977.

Abstract

Animal-infective forms of Trypanosoma brucei (Strain 427) were successfully propagated in HEPES-buffered RPMI 1640 medium in the presence of bovine fibroblast-like cells for over 310 days. The organisms grown in this system were morphologically identical to the long slender bloodstream forms, retained their infectivity for mammalian hosts, and displayed variant-antigen on their surface. Technical details for establishing such bloodstream form cultures are described in the present paper.

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References

    1. Science. 1977 May 27;196(4293):992-4 - PubMed
    1. J Parasitol. 1972 Dec;58(6):1122-36 - PubMed
    1. Parasitology. 1973 Dec;67(3):315-31 - PubMed

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